韦革宏、焦硕等《Plant Cell & Environment》2023年

论文题目：Agricultural tillage practice and rhizosphere selection interactively drive the improvement of soybean plant biomass

论文作者：Shi Chen, Yang Wang, Jiamin Gao, Xingyu Chen, Jiejun Qi, Ziheng Peng, Beibei Chen, Haibo Pan, Chunling Liang, Jiai Liu, Yihe Wang, Gehong Wei*, and Shuo Jiao*

论文摘要：Agriculture systems have been fertilized and cultivated for decades and shape the root-associated microbes of crops, which play key roles in plant growth and productivity. One of the critical issues is revealing the interaction of agricultural management (M) and rhizosphere selection effects (R) on soil microbial communities, root exudates, and plant productivity. Through a field management experiment, high-throughput sequencing, and liquid chromatography mass spectrometry, we investigated the three-way interactions between rhizosphere microbes, root exudates, and plant productivity in two-tillage systems affected by the MxR interaction effect. We found that bacteria were more sensitive to the MxR interaction effect than fungi, and the positive effect of rhizosphere bacterial diversity on plant biomass existed in the bacterial three two-tillage system. In addition, inoculation experiments demonstrated that the nitrogen cycle-related isolate Stenotrophomonas could promote plant growth and alter the activities of extracellular enzymes N-acetyl-D-glucosaminidase and leucine aminopeptidase in rhizosphere soil. Microbe-metabolites network analysis revealed that hubnodes Burkholderia-Caballeronia-Paraburkholderia and Pseudomonas were recruited by specific root metabolites under the MxR interaction effect, and the inoculation of 10 rhizosphere-matched isolates further proved that these microbes could promote the growth of soybean seedlings. KEGG pathway analysis indicated that the growth-promoting mechanisms of these beneficial genera were closely related to metabolic pathways such as amino acid metabolism, melatonin biosynthesis, chitosan biosynthesis, aerobactin biosynthesis, CoA biosynthesis, etc. In summary, these findings provide field observation and experimental evidence for the close relationship between rhizosphere microbes and metabolites, both of which are related to plant productivity under the MxR interaction effect.

农业系统经过几十年的施肥和耕作，塑造了作物根系相关微生物，这些微生物在植物生长中发挥着关键作用。其中一个关键问题是揭示农业管理（M）和根际选择效应（R）对土壤微生物群落、根系分泌物和植物生产力的相互作用。通过田间管理实验、高通量测序和液相色谱-质谱法，我们研究了受MxR相互作用影响的两种耕作系统中根际微生物、根系分泌物和植物生产力之间的三方相互作用。我们发现，细菌对MxR交互效应比真菌更敏感，根际细菌多样性对植物生物量的积极影响存在于细菌的各两耕作系统中。此外，接种试验表明，与氮循环相关的寡养单胞菌可以促进植物生长，并改变根际土壤中胞外酶N-乙酰-D-葡糖苷酶和亮氨酸氨基肽酶的活性。微生物-代谢物网络分析表明，在MxR相互作用作用下，特定根际代谢物招募了伯克霍尔德菌和假单胞菌，接种10株根际来源菌株进一步证明了这些有益微生物可以促进大豆幼苗的生长。KEGG通路分析表明，这些有益微生物的生长促进机制与氨基酸代谢、褪黑素生物合成、壳聚糖生物合成和辅酶A生物合成等代谢途径密切相关。这些发现为根际微生物和代谢物之间的紧密关联提供了现场观察和实验证据，这两者都与MxR相互作用下的植物生产力有关。

论文链接：https://doi.org/10.1111/pce.14694