生物大分子团队《Nature Plants》2024年

论文题目：The catalytic triad of rice NARROW LEAF1 involves H234

论文作者：Ling-Yun Huang, Na-Nv Liu, Wei-Fei Chen, Xia Ai, Hai-Hong Li, Ze-Lin Zhang, Xi-Miao Hou, Philippe Fossé, Olivier Mauffret,Dong-Sheng Lei*, Stephane Rety* & Xu-Guang Xi*

论文摘要：NARROW LEAF1 (NAL1) exerts a multifaceted influence on leaf morphology and crop yield. Recent crystal study proposed that histidine 233 (H233) is part of the catalytic triad. Here we report that unlike suggested previously, H234 instead of H233 is a component of the catalytic triad alongside residues D291 and S385 in NAL1. Remarkably, residue 233 unexpectedly plays a pivotal role in regulating NAL1’s proteolytic activity. These findings establish a strong foundation for utilizing NAL1 in breeding programs aimed at improving crop yield.

窄叶基因 NARROW LEAF1 编码的蛋白NARROW LEAF1（NAL1）对叶片形态和作物产量具有多方面的影响。最近晶体结果显示，第233位组氨酸 (H233)是催化三元体的组成部分。但是，我们发现不同的结果：第234位组氨酸（H234）而非H233是催化三元组的一个组成部分。结果显示H234与残基D291和S385一起构成NAL1的催化三联体。值得注意的是，第233位精氨酸残基直接作用于催化三联体的第291位天冬氨酸，导致催化三联体变构，在调节NAL1的蛋白水解活性中起着关键作用。以上发现在育种上为利用 NAL1 基因提高作物产量的提供了精准靶点。

文章链接：https://www.nature.com/articles/s41477-024-01668-1

Doi：10.1038/s41477-024-01668-1